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fusion solo 6s edge system  (VILBER GmbH)


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    Structured Review

    VILBER GmbH fusion solo 6s edge system
    Fusion Solo 6s Edge System, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 99/100, based on 2972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+solo+6s+edge/FUSION+SOLO+EDGE/pm41943039-66-7-10
    Average 99 stars, based on 2972 article reviews
    fusion solo 6s edge system - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: NVP-BEZ235 enhances autophagy and ameliorates cognitive deficits by targeting tauopathies.
    Article Snippet: .. Protein expression was detected using the West Pico PLUS Chemiluminescent Substrate (Thermo Fisher) and visualized using FUSION Solo 6S EDGE (Vilber Lourmat Sté, Collégien, France). .. The band intensity was quantified using ImageJ software (Bethesda, MD, USA).

    Article Title: Horse Meat Hydrolysate Ameliorates Dexamethasone-Induced Muscle Atrophy in C57BL/6 Mice via the AKT/FoxO3a/mTOR Pathway
    Article Snippet: .. Protein bands were visualized using enhanced chemiluminescence (Cyanagen, Bologna, Italy) and the expression level was analyzed by detecting the density of the band using Fusion Solo 6S Edge (Vilber-Lourmat, Marne la Vallée, France). .. The GA muscle tissues were isolated, homogenized using easy-BLUETM (iNtRON, Republic of Korea), and total RNA was extracted with an RNeasy Mini kit (Qiagen, Hilden, Germany) following the manufacturer’s guidelines. cDNA was synthesized with Pre-Mix (dT18 Plus) (BioFact, Daejeon, Republic of Korea) and subsequently amplified using the LightCycler 96 System (Roche Diagnostics, Basel, Switzerland) alongside 2X Real-Time PCR Master Mix (BioFact). β-actin served as the reference gene to normalize mRNA levels. lists the primers and the corresponding annealing temperatures used for the amplification process.

    Article Title: Horse Meat Hydrolysate Ameliorates Dexamethasone-Induced Muscle Atrophy in C57BL/6 Mice via the AKT/FoxO3a/mTOR Pathway.
    Article Snippet: .. Protein bands were visualized using enhanced chemiluminescence (Cyanagen, Bologna, Italy) and the expression level was analyzed by detecting the density of the band using Fusion Solo 6S Edge (Vilber-Lourmat, Marne la Vallée, France). .. The GA muscle tissues were isolated, homogenized using easy-BLUETM (iNtRON, Republic of Korea), and total RNA was extracted with an RNeasy Mini kit (Qiagen, Hilden, Germany) following the manufacturer’s guidelines. cDNA was synthesized with Pre-Mix (dT18 Plus) (BioFact, Daejeon, Republic of Korea) and subsequently amplified using the LightCycler 96 System (Roche Diagnostics, Basel, Switzerland) alongside 2X Real-Time PCR Master Mix (BioFact). β-actin served as the reference gene to normalize mRNA levels.

    Imaging:

    Article Title: Quercetin and Kaempferol Mitigate Endotoxin‐Induced Skeletal Muscle Wasting by Inhibiting <scp>KLF15</scp> Expression and Restoring the Antioxidant System
    Article Snippet: The loss of muscle mass or muscle atrophy is a significant health concern associated with aging, diabetes, cardiovascular diseases, environmental toxicity, and inflammation, and it is becoming a significant issue requiring effective therapeutic strategies.. Sepsis is caused by bacterial infection leading to inflammation and has a plethora of consequences, one of which is loss of muscle mass commonly observed for intensive care unit patients with a high morbidity rate.. Flavonoids are wellknown natural compounds for restoring muscle wasting.

    Microscopy:

    Article Title: Tanshinone VI Induces Cardiac Differentiation of Cardiosphere-Derived Cells Prepared from Rat Hearts
    Article Snippet: Then they were incubated with anti-rabbit immunoglobulin G (IgG) Dylight488 (Vector Laboratories, Newark, CA, U.S.A.) for 1 h. 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Life Technologies Corp., Carlsbad, CA, U.S.A.) was used for nuclear staining. .. Fluorescent images were captured using a CCD camera (DP80, Olympus, Tokyo, Japan) mounted on an Olympus BX52 microscope, as described previously.21,22) Western Blotting and Detection of Proteins Western blotting was performed as previously described.23,24) In the present study, ECL prime or ECL select (Cytiva, Tokyo, Japan) was used for chemiluminescence quantification, followed by image acquisition using a Fusion SOLO 6S EDGE (Vilber Lourmat, Marne-la-Vallée, France). .. Anti-Islet-1 (H00003670-M05, 1 : 1000, Abnova, Taipei, Taiwan), anti-Nkx2.5 (GTX105711, 1 : 1000, Genetex), anti-GATA4 (36966, 1 : 1000, Cell Signaling Technology, Inc., Danvers, MA, U.S.A.), anti-MEF2c (AV37342, 1 : 1000, Sigma-Aldrich, St. Louis, MO, U.S.A.), anti-α-actinin (A7811, 1 : 1000, Sigma-Aldrich), anti-TNNT2 (GTX62263, 1 : 1000, Genetex), anti-Akt (9272, 1 : 1000, Cell Signaling Technology, Inc.), anti-phospho-Akt Ser473 (12694, 1 : 1000, Cell Signaling Technology, Inc.), anti-glycogen synthase kinase 3α (GSK3α)/β (ADI-KAM-ST002, 1 : 1000, Enzo Life Science Inc., Farmingdale, NY, U.S.A.), antiphospho-GSK3β Ser9 (A00193-100, 1 : 1000, GenScript, Piscataway, NJ, U.S.A.), anti-β-catenin (610153, 1 : 1000, BD Biosciences, San Jose, CA, U.S.A.), anti-phospho-c-Raf Ser338 (9427, 1 : 1000, Cell Signaling Technology, Inc.), antic-Raf (610151, 1 : 1000, BD Biosciences), anti-phospho-MAP kinase kinase (MEK) 1/2 Ser217/Ser221 (8211, 1 : 1000, Cell Signaling Technology, Inc.), anti-MEK1/2 (8727, 1 : 1000, Cell Signaling Technology, Inc.), anti-phospho-extracellular signalregulated kinase 1/2 (ERK1/2) Thr202/Tyr204 (9101, 1 : 1000, Cell Signaling Technology, Inc.), anti-ERK1/2 (9102, 1 : 1000, Cell Signaling Technology, Inc.), and anti-GAPDH (MAB374, 1 : 10000, Sigma-Aldrich) antibodies were used.

    Western Blot:

    Article Title: Tanshinone VI Induces Cardiac Differentiation of Cardiosphere-Derived Cells Prepared from Rat Hearts
    Article Snippet: Then they were incubated with anti-rabbit immunoglobulin G (IgG) Dylight488 (Vector Laboratories, Newark, CA, U.S.A.) for 1 h. 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Life Technologies Corp., Carlsbad, CA, U.S.A.) was used for nuclear staining. .. Fluorescent images were captured using a CCD camera (DP80, Olympus, Tokyo, Japan) mounted on an Olympus BX52 microscope, as described previously.21,22) Western Blotting and Detection of Proteins Western blotting was performed as previously described.23,24) In the present study, ECL prime or ECL select (Cytiva, Tokyo, Japan) was used for chemiluminescence quantification, followed by image acquisition using a Fusion SOLO 6S EDGE (Vilber Lourmat, Marne-la-Vallée, France). .. Anti-Islet-1 (H00003670-M05, 1 : 1000, Abnova, Taipei, Taiwan), anti-Nkx2.5 (GTX105711, 1 : 1000, Genetex), anti-GATA4 (36966, 1 : 1000, Cell Signaling Technology, Inc., Danvers, MA, U.S.A.), anti-MEF2c (AV37342, 1 : 1000, Sigma-Aldrich, St. Louis, MO, U.S.A.), anti-α-actinin (A7811, 1 : 1000, Sigma-Aldrich), anti-TNNT2 (GTX62263, 1 : 1000, Genetex), anti-Akt (9272, 1 : 1000, Cell Signaling Technology, Inc.), anti-phospho-Akt Ser473 (12694, 1 : 1000, Cell Signaling Technology, Inc.), anti-glycogen synthase kinase 3α (GSK3α)/β (ADI-KAM-ST002, 1 : 1000, Enzo Life Science Inc., Farmingdale, NY, U.S.A.), antiphospho-GSK3β Ser9 (A00193-100, 1 : 1000, GenScript, Piscataway, NJ, U.S.A.), anti-β-catenin (610153, 1 : 1000, BD Biosciences, San Jose, CA, U.S.A.), anti-phospho-c-Raf Ser338 (9427, 1 : 1000, Cell Signaling Technology, Inc.), antic-Raf (610151, 1 : 1000, BD Biosciences), anti-phospho-MAP kinase kinase (MEK) 1/2 Ser217/Ser221 (8211, 1 : 1000, Cell Signaling Technology, Inc.), anti-MEK1/2 (8727, 1 : 1000, Cell Signaling Technology, Inc.), anti-phospho-extracellular signalregulated kinase 1/2 (ERK1/2) Thr202/Tyr204 (9101, 1 : 1000, Cell Signaling Technology, Inc.), anti-ERK1/2 (9102, 1 : 1000, Cell Signaling Technology, Inc.), and anti-GAPDH (MAB374, 1 : 10000, Sigma-Aldrich) antibodies were used.



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